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Image Search Results
Journal:
Article Title: CD40-CD154 interactions between macrophages and natural killer cells during sepsis are critical for macrophage activation and are not interferon gamma dependent
doi: 10.1111/j.1365-2249.2004.02547.x
Figure Lengend Snippet: Role of IFN-γ on NK cell and macrophage (MΦ) activation in single cell-type and mixed-cell coculture. CD69 expression was determined on splenic NK cells isolated from IFN-γ–/– mice after 24 h culture alone or with MΦ from wild type (WT) C57BL/6, with and without LPS (a). Cocultures of NK cells with MΦ (NK + MΦ, NK + MΦ + LPS) showed increases in CD69 expression compared to culture alone (NK, NK + LPS) both with and without LPS, although these levels were significantly decreased from CD69 levels in NK cells from WT mice (compare with Fig. 1a). Phagocytosis level was determined at 48 h in WT MΦ with and without NK cells from IFN-γ–/– mice and with and without LPS (b). There were significant increases in phagocytosis mean channel fluorescence (MCF) of MΦ cocultured with NK cells (MΦ + NK, MΦ + NK + LPS), although these levels were again lower than in WT mice (compare with Figs 1b,c). There were no differences in phagocytosis MCF between MΦ cocultured with NK cells with and without 1 µg/ml anti-IFN-γ neutralizing antibody (c). n = 6 per experimental group and data are representative of separate repeated experiments. Data presented as mean ± s.e.m. * or †P < 0·05, Kruskal–Wallis (CD69 percentages), anova (MCF).
Article Snippet: Macrophages were also cultured with NK cells and 1 µ g/ml of neutralizing
Techniques: Activation Assay, Expressing, Isolation, Fluorescence
Journal:
Article Title: CD40-CD154 interactions between macrophages and natural killer cells during sepsis are critical for macrophage activation and are not interferon gamma dependent
doi: 10.1111/j.1365-2249.2004.02547.x
Figure Lengend Snippet: Levels of IL-6 measured by ELISA in culture supernatant from peritoneal macrophages cultured for 48 h with and without splenic NK cells and with and without 1 µ g/ml LPS. Peritoneal macrophages from CD40−/– mice were also used, as well as splenic NK cells from CD154−/– and IFN- γ −/– mice
Article Snippet: Macrophages were also cultured with NK cells and 1 µ g/ml of neutralizing
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture
Journal:
Article Title: CD40-CD154 interactions between macrophages and natural killer cells during sepsis are critical for macrophage activation and are not interferon gamma dependent
doi: 10.1111/j.1365-2249.2004.02547.x
Figure Lengend Snippet: Levels of TNF- α measured by ELISA in culture supernatant from peritoneal macrophages cultured for 48 h with and without splenic NK cells and with and without 1 µ g/ml LPS. Peritoneal macrophages from CD40–/– mice were also used, as well as splenic NK cells from CD154–/– and IFN- γ –/– mice
Article Snippet: Macrophages were also cultured with NK cells and 1 µ g/ml of neutralizing
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture
Journal:
Article Title: CD40-CD154 interactions between macrophages and natural killer cells during sepsis are critical for macrophage activation and are not interferon gamma dependent
doi: 10.1111/j.1365-2249.2004.02547.x
Figure Lengend Snippet: Levels of IL-12 measured by ELISA in culture supernatant from peritoneal macrophages cultured for 48 h with and without splenic NK cells and with and without 1 µ g/ml LPS. Peritoneal macrophages from CD40−/– mice were also used, as well as splenic NK cells from CD154–/– and IFN- γ –/– mice
Article Snippet: Macrophages were also cultured with NK cells and 1 µ g/ml of neutralizing
Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture